1EJW
CRYSTAL STRUCTURE OF WILD-TYPE KLEBSIELLA AEROGENES UREASE AT 298K
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Detector | PRINCETON 2K |
| Spacegroup name | I 21 3 |
| Unit cell lengths | 170.800, 170.800, 170.800 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.900 |
| Rwork | 0.144 |
| R-free | 0.20000 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.132 | 0.339 |
| Number of reflections | 59435 | |
| Completeness [%] | 95.0 | 89 |
| Redundancy | 4.7 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | lithium sulfate, Hepes, EDTA, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
