1EJN

UROKINASE PLASMINOGEN ACTIVATOR B-CHAIN INHIBITOR COMPLEX

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	298
Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	52.850, 54.770, 81.580
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	500.000 - 1.800
R-factor	0.2 *
Rwork	0.200
R-free	0.24000
RMSD bond length	0.005
RMSD bond angle	1.200
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	500.000	2.000
High resolution limit [Å]	1.800	1.800
Rmerge	0.087	0.560
Total number of observations	46430 *
Number of reflections	20187 *
Completeness [%]	84.0	60
Redundancy	2.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	other *	5.2	4 *	pH 5.2, VAPOR DIFFUSION, SITTING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1		1 (M)
2	1	1	ammonium sulfate	0.8 (M)
3	1	1	sodium citrate	0.1 (M)
4	1	2	benzamidine	6 (mM)