1EJL

MOUSE IMPORTIN ALPHA-SV40 LARGE T ANTIGEN NLS PEPTIDE COMPLEX

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	AREA DETECTOR
Collection date	1999-08-27
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	78.228, 89.858, 99.490
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.800
R-factor	0.215
Rwork	0.215
R-free	0.25400
RMSD bond length	0.007
RMSD bond angle	1.200
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (0.4)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.900
High resolution limit [Å]	2.800	2.800
Rmerge	0.159	0.742
Total number of observations	126036 *
Number of reflections	17900	1669 *
<I/σ(I)>	9
Completeness [%]	93.1	95.6
Redundancy	7	5.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	6.5	293	sodium citrate, Hepes, DTT, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	sodium citrate	0.55 (M)
2	1	1	HEPES	100 (mM)
3	1	1	dithiothreitol	10 (mM)