1EJJ
CRYSTAL STRUCTURAL ANALYSIS OF PHOSPHOGLYCERATE MUTASE COCRYSTALLIZED WITH 3-PHOSPHOGLYCERATE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 1999-02-11 |
Detector | BRANDEIS - B4 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 58.486, 206.293, 125.134 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 * - 1.900 |
R-factor | 0.2071 * |
Rwork | 0.207 |
R-free | 0.24700 |
RMSD bond length | 0.007 * |
RMSD bond angle | 1.300 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | X-PLOR (3.851) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.099 | 0.233 |
Number of reflections | 57256 | |
<I/σ(I)> | 6.6 | |
Completeness [%] | 96.2 | 85.1 |
Redundancy | 3.8 | 2.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 22 * | Chander, M., (1999) J.Struct.Biol., 126, 156. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 50 (mg/ml) | |
2 | 1 | drop | 3-PGA | 150 (mM) | |
3 | 1 | reservoir | ammonium sulfate | 2.0 (M) | |
4 | 1 | reservoir | zinc acetate | 25 (mM) | |
5 | 1 | reservoir | 20 (mM) | ||
6 | 1 | reservoir | 2-mercaptoethanol | 15 (mM) | |
7 | 1 | reservoir | PEG200 | 3 (%) | |
8 | 1 | reservoir | Tris-HCl | 50 (mM) |