1EJF
CRYSTAL STRUCTURE OF THE HUMAN CO-CHAPERONE P23
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 1998-12-31 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 61.802, 61.802, 162.888 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 * - 2.500* |
| R-factor | 0.236 * |
| Rwork | 0.236 |
| R-free | 0.26800 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 27.500 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MLPHARE |
| Refinement software | CNS (0.9) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 * | 2.620 |
| High resolution limit [Å] | 2.490 | 2.490 |
| Rmerge | 0.050 | 0.074 |
| Total number of observations | 97860 * | |
| Number of reflections | 11743 * | 1651 * |
| <I/σ(I)> | 7.7 | |
| Completeness [%] | 99.4 | 98.8 |
| Redundancy | 8.3 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 298 | ammonium sulfate, bicine, tris, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | ammonium sulfate | 1.9-2.1 (M) | |
| 2 | 1 | reservoir | Bicine | 0.1 (M) | pH9.0 |
| 3 | 1 | drop | protein | 20 (mg/ml) | |
| 4 | 1 | drop | Tris-HCl | 10 (mM) | pH7.5 |
