1EJ3
CRYSTAL STRUCTURE OF AEQUORIN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X8C |
| Synchrotron site | NSLS |
| Beamline | X8C |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 1999-05-05 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 81.270, 81.270, 163.660 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.300 |
| R-factor | 0.221 |
| Rwork | 0.219 |
| R-free | 0.25300 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 19.600 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (0.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.440 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.068 | 0.370 |
| Number of reflections | 23141 | |
| <I/σ(I)> | 24.7 | |
| Completeness [%] | 92.5 | 69.8 |
| Redundancy | 10.1 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 17 * | drop consists of equal volume of protein and reservoir solutions * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | EDTA | 2 (mM) | |
| 3 | 1 | drop | ammonium sulfate | 1.2 (M) | |
| 4 | 1 | drop | Bis-Tris | 10 (mM) | |
| 5 | 1 | reservoir | ammonium sulfate | 63 (%sat) | |
| 6 | 1 | reservoir | Bis-Tris | 10 (mM) |
