1EIZ
FTSJ RNA METHYLTRANSFERASE COMPLEXED WITH S-ADENOSYLMETHIONINE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 160 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-02-02 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 36.457, 65.867, 72.877 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 18.220 - 1.700 |
| R-factor | 0.191 * |
| Rwork | 0.190 |
| R-free | 0.23700 |
| Structure solution method | MIR |
| RMSD bond length | 0.013 |
| RMSD bond angle | 24.400 * |
| Data reduction software | bioteX |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | CNS (0.9) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.058 | 0.371 |
| Number of reflections | 24364 | |
| <I/σ(I)> | 14 | |
| Completeness [%] | 94.0 | 62.1 |
| Redundancy | 7 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 5.7 * | 298 | 1mM AdoMet, 33% PEG 4000, 0.19 M ammonium acetate, 0.1 M sodium citrate pH 5.65, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | FtsJ | 12 (mg/ml) | |
| 2 | 1 | drop | AdoMet | 1 (mM) | |
| 3 | 1 | reservoir | PEG4000 | 33 (%) | |
| 4 | 1 | reservoir | ammonium acetate | 0.19 (M) | |
| 5 | 1 | reservoir | sodium citrate | 0.1 (M) |
