1EIZ

FTSJ RNA METHYLTRANSFERASE COMPLEXED WITH S-ADENOSYLMETHIONINE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	160
Detector technology	IMAGE PLATE
Collection date	1998-02-02
Detector	RIGAKU RAXIS IV
Spacegroup name	P 21 21 21
Unit cell lengths	36.457, 65.867, 72.877
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	18.220 - 1.700
R-factor	0.191 *
Rwork	0.190
R-free	0.23700
Structure solution method	MIR
RMSD bond length	0.013
RMSD bond angle	24.400 *
Data reduction software	bioteX
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	CNS (0.9)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.530
High resolution limit [Å]	1.500	1.500
Rmerge	0.058	0.371
Number of reflections	24364
<I/σ(I)>	14
Completeness [%]	94.0	62.1
Redundancy	7	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	5.7 *	298	1mM AdoMet, 33% PEG 4000, 0.19 M ammonium acetate, 0.1 M sodium citrate pH 5.65, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	FtsJ	12 (mg/ml)
2	1	drop	AdoMet	1 (mM)
3	1	reservoir	PEG4000	33 (%)
4	1	reservoir	ammonium acetate	0.19 (M)
5	1	reservoir	sodium citrate	0.1 (M)