1EIY

THE CRYSTAL STRUCTURE OF PHENYLALANYL-TRNA SYNTHETASE FROM THERMUS THERMOPHILUS COMPLEXED WITH COGNATE TRNAPHE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	293
Detector technology	AREA DETECTOR
Collection date	1992-10-10
Detector	XENTRONICS
Spacegroup name	P 32 2 1
Unit cell lengths	175.000, 175.000, 140.600
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.000 - 3.300
R-factor	0.221 *
Rwork	0.221
R-free	0.28700
RMSD bond length	0.025
RMSD bond angle	2.300
Data reduction software	DENZO
Data scaling software	X-GEN
Phasing software	X-PLOR
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.000	3.400
High resolution limit [Å]	3.280 *	3.300
Rmerge	0.158	0.338
Number of reflections	33340
Completeness [%]	86.3	48

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.2	293	Reshetnikova, L., (1993) J. Mol. Biol., 231, 927. *
1	VAPOR DIFFUSION, HANGING DROP	7.2	293	Reshetnikova, L., (1993) J. Mol. Biol., 231, 927. *

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	MgSO4
2	1	1	(NH4)2SO4
3	1	2	MgSO4
4	1	2	(NH4)2SO4