1EIR

2,3-DIHYDROXYBIPHENYL-1,2-DIOXYGENASE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1998-10-29
Detector	RIGAKU RAXIS IIC
Spacegroup name	I 4 2 2
Unit cell lengths	121.600, 121.600, 108.700
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	60.000 - 2.000
R-factor	0.186
Rwork	0.181
R-free	0.24400 *
RMSD bond length	0.012 *
RMSD bond angle	2.500 *
Phasing software	AMoRE
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	60.000	2.250
High resolution limit [Å]	1.980	1.980
Rmerge	0.083	0.208
Number of reflections	22454 *
<I/σ(I)>	10.3
Completeness [%]	80.8 *	58.6 *
Redundancy	2.1	1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Batch method	7.5	12 *	285K, Tris/HCl, Ammonium sulfate, hexylene glycol, pH 7.5, Batch method

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	10 (mg/ml)
2	1	1	Tris-HCl	100 (mM)
3	1	1	ammonium sulfate	27.7 (%sat)
4	1	1	hexylene glycol	18 (%(v/v))