1EIF
EUKARYOTIC TRANSLATION INITIATION FACTOR 5A FROM METHANOCOCCUS JANNASCHII
Experimental procedure
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X12B |
Synchrotron site | NSLS |
Beamline | X12B |
Temperature [K] | 290 |
Detector technology | IMAGE PLATE |
Collection date | 1997-11 |
Detector | MARRESEARCH |
Spacegroup name | P 41 2 2 |
Unit cell lengths | 45.520, 45.520, 155.590 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 6.000 - 1.900 |
R-factor | 0.205 |
Rwork | 0.205 |
R-free | 0.28900 |
Structure solution method | MIR, MAD |
RMSD bond length | 0.012 |
RMSD bond angle | 30.030 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR (3.85) |
Refinement software | X-PLOR (3.85) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.930 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.039 | 0.326 |
Number of reflections | 12622 | |
<I/σ(I)> | 24.1 | 5.3 |
Completeness [%] | 92.3 | 97.7 |
Redundancy | 4.02 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 4.6 | 0.1 M SODIUM ACETATE (PH 4.6) 8% PEK4000 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | drop | sodium acetate | 0.1 (M) | |
3 | 1 | drop | PEG4000 | 8 (%(w/v)) |