1EIE
CRYSTAL STRUCTURE OF F120W MUTANT OF BOVINE PANCREATIC RIBONUCLEASE A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44B2 |
Synchrotron site | SPring-8 |
Beamline | BL44B2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 1999-06-03 |
Detector | ADSC QUANTUM 4r |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 63.900, 63.900, 63.330 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 6.000 - 1.400 |
R-factor | 0.218 * |
Rwork | 0.218 |
R-free | 0.27200 |
RMSD bond length | 0.005 |
RMSD bond angle | 1.200 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((TRUNCATE)) |
Phasing software | X-PLOR |
Refinement software | X-PLOR (3.851) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 16.800 | 1.008 |
High resolution limit [Å] | 1.000 | 1.000 |
Rmerge | 0.060 | |
Total number of observations | 886320 * | |
Number of reflections | 80099 | |
<I/σ(I)> | 20.89 | |
Completeness [%] | 99.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 25 * | ammonium sulfate, sodium chloride, sodium acetate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 24 (mg/ml) | |
2 | 1 | reservoir | sodium acetate | 100 (mM) | pH6.0 |
3 | 1 | reservoir | ammonium sulfate | 35 (%(v/v)saturated) | |
4 | 1 | reservoir | 50 (%(v/v)saturated) |