1EIA
X-RAY CRYSTAL STRUCTURE OF EQUINE INFECTIOUS ANEMIA VIRUS (EIAV) CAPSID PROTEIN P26
Experimental procedure
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X12C |
Synchrotron site | NSLS |
Beamline | X12C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 1997-11-17 |
Detector | BRANDEIS |
Spacegroup name | I 4 3 2 |
Unit cell lengths | 176.760, 176.760, 176.760 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.000 - 2.700 |
R-factor | 0.227 |
Rwork | 0.227 |
R-free | 0.27500 |
Structure solution method | TREAT MAD AS MIR |
RMSD bond length | 0.009 |
RMSD bond angle | 18.300 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASES |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.000 | 2.800 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.086 | 0.357 * |
Number of reflections | 12292 * | |
<I/σ(I)> | 40 | 6 |
Completeness [%] | 99.4 | 94.6 |
Redundancy | 17 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 6.5 | pH 6.5 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 22 (mg/ml) | |
2 | 1 | drop | sodium phosphate | 1 (mM) | |
3 | 1 | reservoir | sodium citrate | 0.1 (M) | |
4 | 1 | reservoir | PEG3350 | 10 (%(w/v)) | |
5 | 1 | reservoir | isopropanol | 15 (%(v/v)) |