1EIA
X-RAY CRYSTAL STRUCTURE OF EQUINE INFECTIOUS ANEMIA VIRUS (EIAV) CAPSID PROTEIN P26
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1997-11-17 |
| Detector | BRANDEIS |
| Spacegroup name | I 4 3 2 |
| Unit cell lengths | 176.760, 176.760, 176.760 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.000 - 2.700 |
| R-factor | 0.227 |
| Rwork | 0.227 |
| R-free | 0.27500 |
| Structure solution method | TREAT MAD AS MIR |
| RMSD bond length | 0.009 |
| RMSD bond angle | 18.300 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASES |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.086 | 0.357 * |
| Number of reflections | 12292 * | |
| <I/σ(I)> | 40 | 6 |
| Completeness [%] | 99.4 | 94.6 |
| Redundancy | 17 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.5 | pH 6.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 22 (mg/ml) | |
| 2 | 1 | drop | sodium phosphate | 1 (mM) | |
| 3 | 1 | reservoir | sodium citrate | 0.1 (M) | |
| 4 | 1 | reservoir | PEG3350 | 10 (%(w/v)) | |
| 5 | 1 | reservoir | isopropanol | 15 (%(v/v)) |
