1EHZ
The crystal structure of yeast phenylalanine tRNA at 1.93 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.981, 33.389, 61.921 |
| Unit cell angles | 90.00, 90.20, 90.00 |
Refinement procedure
| Resolution | 40.000 - 1.930 |
| R-factor | 0.2334 * |
| Rwork | 0.233 |
| R-free | 0.25300 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.605 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | |
| High resolution limit [Å] | 1.800 | |
| Rmerge | 0.057 | 0.285 * |
| Number of reflections | 19877 | |
| <I/σ(I)> | 22.4 | |
| Completeness [%] | 96.5 | 83.3 * |
| Redundancy | 14 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | isopropanol, MgCl2, spermine, cacodylate, pH 6 to 7, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MgCl2 | ||
| 2 | 1 | 1 | spermine | ||
| 3 | 1 | 1 | cacodylate | ||
| 4 | 1 | 1 | isopropanol | ||
| 5 | 1 | 2 | isopropanol |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 4 (mg/ml) | |
| 2 | 1 | reservoir | isopropanol | 7-9 (%) | |
| 3 | 1 | reservoir | 40 (mM) | ||
| 4 | 1 | reservoir | spermine | 3 (mM) | |
| 5 | 1 | reservoir | sodium cacodylate | 40 (mM) |
