1EHL
64M-2 ANTIBODY FAB COMPLEXED WITH D(5HT)(6-4)T
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-07-26 |
| Detector | RIGAKU |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 144.500, 48.062, 77.939 |
| Unit cell angles | 90.00, 104.69, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.400 |
| R-factor | 0.199 |
| Rwork | 0.192 |
| R-free | 0.27900 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.871 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.843) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.700 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.084 | 0.221 |
| Total number of observations | 77929 * | |
| Number of reflections | 18790 | 1571 * |
| <I/σ(I)> | 18.5 | |
| Completeness [%] | 93.5 | 79.1 |
| Redundancy | 4.1 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 20 * | PEG 8000, nickel chloride, HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | 64M-2 Fab | 0.2 (mM) | |
| 2 | 1 | drop | dT(6-4)T | 0.25 (mM) | |
| 3 | 1 | reservoir | PEG8000 | 14-18 (%(w/v)) | |
| 4 | 1 | reservoir | HEPES-Na | 0.1 (M) | or Tris-HCl, pH7.5-8.4 |
| 5 | 1 | reservoir | 10 (mM) |
