1EHC

STRUCTURE OF SIGNAL TRANSDUCTION PROTEIN CHEY

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	290
Detector technology	AREA DETECTOR
Detector	SIEMENS
Spacegroup name	P 21 21 21
Unit cell lengths	45.850, 47.030, 54.040
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.260
R-factor	0.143 *
Rwork	0.143
Structure solution method	PHASED WITH WT
Starting model (for MR)	MODIFIED WILD-TYPE CHEY
RMSD bond length	0.016
RMSD bond angle	0.051
Data reduction software	XENGEN
Data scaling software	XENGEN
Refinement software	PROFFT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]		2.370
High resolution limit [Å]	2.260	2.260
Rmerge	0.060
Total number of observations	12828 *
Number of reflections	5108
Completeness [%]	87.8	24.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Microdialysis *	8.3	4 *	Volz, K., (1986) J.Biol.Chem., 261, 4723. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	ammonium salfate	2.7 (M)
2	1	1	Tris-HCl	50 (mM)