1EHB

CRYSTAL STRUCTURE OF RECOMBINANT TRYPSIN-SOLUBILIZED FRAGMENT OF CYTOCHROME B5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SEALED TUBE
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	1999-03-30
Detector	MARRESEARCH
Spacegroup name	C 1 2 1
Unit cell lengths	70.700, 40.440, 39.280
Unit cell angles	90.00, 111.76, 90.00

Refinement procedure

Resolution	100.000 - 1.900
R-factor	0.198
Rwork	0.198
R-free	0.24800
RMSD bond length	0.010
RMSD bond angle	1.100
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS (0.9)
Refinement software	CNS (0.9)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	1.940
High resolution limit [Å]	1.900	1.900
Rmerge	0.060	0.210
Number of reflections	7783
<I/σ(I)>	18.2
Completeness [%]	94.3	92.6
Redundancy	3.3	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	20 *	phosphate buffer, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	phosphate	3.2 (M)	pH8.0
2	1	drop	protein	20 (mg/ml)