1EH7
METHYLATED HUMAN O6-ALKYLGUANINE-DNA ALKYLTRANSFERASE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL7-1 |
Synchrotron site | SSRL |
Beamline | BL7-1 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1999-03-16 |
Detector | MARRESEARCH |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 71.401, 71.401, 76.325 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 18.230 - 2.000 |
R-factor | 0.199 * |
Rwork | 0.199 |
R-free | 0.22900 |
Starting model (for MR) | PDB 1EH6 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.400 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.051 | 0.255 |
Total number of observations | 28548 * | |
Number of reflections | 15195 | |
<I/σ(I)> | 13.4926 | 3.133 |
Completeness [%] | 97.2 | 98.2 * |
Redundancy | 1.472 | 1.858 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 25 * | 18 MG/ML PROTEIN, 1.5 M SUCROSE, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 18 (mg/ml) | |
2 | 1 | reservoir | sucrose | 1.5 (M) |