1EH1

RIBOSOME RECYCLING FACTOR FROM THERMUS THERMOPHILUS

Experimental procedure

Experimental method	MAD
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	110
Detector technology	IMAGE PLATE
Collection date	1999-07-30
Detector	RIGAKU
Spacegroup name	P 32 2 1
Unit cell lengths	71.500, 71.500, 79.600
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 - 2.600
R-factor	0.236
Rwork	0.234
R-free	0.30700
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	1.100
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.660
High resolution limit [Å]	2.600	2.600
Rmerge	0.038	0.234
Total number of observations	28513 *
Number of reflections	7568 *
<I/σ(I)>	17.6
Completeness [%]	99.7	98.7
Redundancy	3.8	1.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6 *	293	ammonium sulphate, sodium acetate, MES, pH 4.9, VAPOR DIFFUSION, HANGING DROP, temperature 20K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10-15 (mg/ml)
2	1	reservoir	ammonium sulfate	2 (M)
3	1	reservoir	sodium acetate trihydrate	0.1 (M)