1EGU
CRYSTAL STRUCTURE OF STREPTOCOCCUS PNEUMONIAE HYALURONATE LYASE AT 1.56 A RESOLUTION
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | BRANDEIS - B4 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 84.041, 104.279, 101.763 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.560 |
| R-factor | 0.2 * |
| Rwork | 0.200 |
| R-free | 0.23600 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 2.380 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | XTALVIEW |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.620 |
| High resolution limit [Å] | 1.560 | 1.560 |
| Rmerge | 0.041 | 0.204 |
| Total number of observations | 411697 * | |
| Number of reflections | 124919 | |
| <I/σ(I)> | 16.1 | |
| Completeness [%] | 98.6 | 96 |
| Redundancy | 3.4 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 * | 295 | Jadrzejas, M.J., (1998) J. Struct. Biol., 121, 73. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Tris-HCl | 10.0 (mM) | |
| 2 | 1 | drop | EDTA | 2.0 (mM) | |
| 3 | 1 | drop | enzyme | 5 (mg/ml) | |
| 4 | 1 | reservoir | ammonium sulfate | 3.2 (M) | |
| 5 | 1 | reservoir | sodium cacodylate | 100 (mM) |
