1EGK
CRYSTAL STRUCTURE OF A NUCLEIC ACID FOUR-WAY JUNCTION
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-03-20 |
| Detector | MARRESEARCH |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 62.810, 114.260, 171.450 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 3.100 |
| R-factor | 0.2296 |
| Rwork | 0.226 |
| R-free | 0.25800 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 28.900 * |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | SOLVE |
| Refinement software | X-PLOR (3.843) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 3.100 |
| Rmerge | 0.051 |
| Number of reflections | 10948 |
| <I/σ(I)> | 7.1 |
| Completeness [%] | 99.2 |
| Redundancy | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 6 | 297.5 | 50MM SODIUM CACODYLATE 6.0, 20MM MAGNESIUM CHLORIDE, 1MM SPERMINE, 25% MPD, VAPOR DIFFUSION, SITTING DROP, temperature 24.5K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | SODIUM CACODYLATE | ||
| 2 | 1 | 1 | SPERMINE | ||
| 3 | 1 | 1 | MGCL2 | ||
| 4 | 1 | 1 | MPD | ||
| 5 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | sodium cacodylate | 50 (mM) | |
| 2 | 1 | reservoir | 20 (mM) | ||
| 3 | 1 | reservoir | spermine | 1 (mM) | |
| 4 | 1 | reservoir | 25 (%(v/v)) |
