1EGG

STRUCTURE OF A C-TYPE CARBOHYDRATE-RECOGNITION DOMAIN (CRD-4) FROM THE MACROPHAGE MANNOSE RECEPTOR

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-1
Synchrotron site	SSRL
Beamline	BL9-1
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1997-05-15
Detector	MARRESEARCH
Spacegroup name	C 1 2 1
Unit cell lengths	129.890, 38.280, 54.810
Unit cell angles	90.00, 99.09, 90.00

Refinement procedure

Resolution	500.000 - 2.300
R-factor	0.216 *
Rwork	0.216
R-free	0.27800
RMSD bond length	0.006
RMSD bond angle	1.400
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (0.5)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.350
High resolution limit [Å]	2.300	2.300
Rmerge	0.068	0.259
Total number of observations	43029 *
Number of reflections	11926
Completeness [%]	98.2	98.4
Redundancy	3.6	1.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	21 *	8-13% polyethylene glycol, 100 mM Tris pH 8.0, 100 mM NaCl, 10 mM CaCl2, 45 mM MgCl2, VAPOR DIFFUSION, HANGING DROP, temperature 294K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	12 (mg/ml)
2	1	drop		3-5 (mM)
3	1	reservoir	PEG10000	8-13 (%)
4	1	reservoir	Tris	100 (mM)
5	1	reservoir		100 (mM)
6	1	reservoir		10 (mM)