1EGE

STRUCTURE OF T255E, E376G MUTANT OF HUMAN MEDIUM CHAIN ACYL-COA DEHYDROGENASE

Experimental procedure

Detector technology	IMAGE PLATE
Collection date	1993-06-21
Detector	RIGAKU
Spacegroup name	P 43 21 2
Unit cell lengths	169.590, 169.590, 151.100
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.750
R-factor	0.221
Rwork	0.221
RMSD bond length	0.009
RMSD bond angle	22.300 *
Data reduction software	R-AXIS (IIC)
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	2.750 *
Rmerge	0.107 *
Total number of observations	104904 *
Number of reflections	44572
Completeness [%]	79.0
Redundancy	2.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	7 *	4 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	enzyme		0.027mg
2	1	drop	Tris acetate	140 (mM)
3	1	drop	PEG8000	8.0 (%(w/v))
4	1	reservoir	PEG4000	50 (%)