1EG4
STRUCTURE OF A DYSTROPHIN WW DOMAIN FRAGMENT IN COMPLEX WITH A BETA-DYSTROGLYCAN PEPTIDE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 213 |
| Detector technology | CCD |
| Collection date | 1999-04-12 |
| Detector | APS-1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 48.680, 67.050, 83.840 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.000 |
| R-factor | 0.197 * |
| Rwork | 0.197 |
| R-free | 0.24900 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.654 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | DM |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.052 | 0.295 |
| Total number of observations | 120237 * | |
| Number of reflections | 21895 | |
| <I/σ(I)> | 12.6 | |
| Completeness [%] | 98.3 | 95.3 |
| Redundancy | 5.5 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 * | 22 * | ammonium sulfate, glycerol, DTT, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | MOPS | 20 (mM) | |
| 3 | 1 | drop | 100 (mM) | ||
| 4 | 1 | drop | dithiothreitol | 5 (mM) | |
| 5 | 1 | reservoir | HEPES | 100 (mM) | |
| 6 | 1 | reservoir | ammonium sulfate | 1.0 (M) | |
| 7 | 1 | reservoir | glycerol | 10 (%(v/v)) | |
| 8 | 1 | reservoir | dithiothreitol | 5 (mM) |
