1EG2

CRYSTAL STRUCTURE OF RHODOBACTER SPHEROIDES (N6 ADENOSINE) METHYLTRANSFERASE (M.RSRI)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X12C
Synchrotron site	NSLS
Beamline	X12C
Temperature [K]	93
Detector technology	CCD
Collection date	1998-06-05
Detector	BRANDEIS
Spacegroup name	C 2 2 21
Unit cell lengths	70.420, 130.253, 67.284
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.210 - 1.750
Rwork	0.214
R-free	0.24000 *
RMSD bond length	0.006 *
RMSD bond angle	1.150 *
Data reduction software	MARMAD
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000 *	1.810
High resolution limit [Å]	1.750 *	1.750
Rmerge	0.035	0.242
Total number of observations	69339 *
Number of reflections	20741 *	2281 *
<I/σ(I)>	13.2
Completeness [%]	94.2	93.9
Redundancy	5.6	2.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	7.4 *	291	1.5 M Li2SO4, 0.1 M HEPES, 0.5% NaAzide, 10 mM EDTA, pH 7.6, VAPOR DIFFUSION, HANGING DROP, temperature 291K
1	unknown *	7.4 *	291	1.5 M Li2SO4, 0.1 M HEPES, 0.5% NaAzide, 10 mM EDTA, pH 7.6, VAPOR DIFFUSION, HANGING DROP, temperature 291K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1		1.5 (M)
2	1	1	HEPES	100 (mM)
3	1	1	EDTA	10 (mM)