1EG1
ENDOGLUCANASE I FROM TRICHODERMA REESEI
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 278 |
| Detector technology | IMAGE PLATE |
| Collection date | 1992-11 |
| Detector | MARRESEARCH |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 101.400, 101.400, 199.500 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 3.600 |
| R-factor | 0.201 |
| Rwork | 0.201 |
| R-free | 0.25800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | HUMICOLA INSOLENS EG1 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.200 * |
| Data reduction software | DENZO |
| Data scaling software | CCP4 |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (4.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 71.100 | 3.690 |
| High resolution limit [Å] | 3.600 | 3.600 |
| Rmerge | 0.117 | 0.404 |
| Total number of observations | 45852 * | |
| Number of reflections | 11696 | 3353 * |
| Completeness [%] | 92.7 | 93.3 |
| Redundancy | 3.9 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 4.5 | drop contains equal volume of protein and reservoir solutions * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | glucose | 100 (mM) | |
| 3 | 1 | reservoir | ammonium sulfate | 1 (M) | |
| 4 | 1 | reservoir | ammonium citrate | 100 (mM) |
