1EFW
Crystal structure of aspartyl-tRNA synthetase from Thermus thermophilus complexed to tRNAasp from Escherichia coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LURE BEAMLINE DW32 |
| Synchrotron site | LURE |
| Beamline | DW32 |
| Temperature [K] | 123 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Spacegroup name | P 63 |
| Unit cell lengths | 251.450, 251.450, 88.700 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 15.000 - 3.000 |
| R-factor | 0.246 * |
| Rwork | 0.248 |
| R-free | 0.29100 * |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.820 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.088 | 0.239 |
| Number of reflections | 58634 | |
| <I/σ(I)> | 14.9 | |
| Completeness [%] | 91.9 | 98.3 |
| Redundancy | 3.9 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7.5 | 17 * | sodium citrate, magnesium chloride, Na-HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 290.0K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | sodium citrate | ||
| 2 | 1 | 1 | MgCl2 | ||
| 3 | 1 | 1 | Na-HEPES | ||
| 4 | 1 | 2 | sodium citrate | ||
| 5 | 1 | 2 | MgCl2 | ||
| 6 | 1 | 2 | Na-HEPES |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15 (mg/ml) | |
| 2 | 1 | drop | tRNA | 7.35 (mg/ml) | |
| 3 | 1 | reservoir | sodium citrate | 0.7 (M) | |
| 4 | 1 | reservoir | 10 (mM) | ||
| 5 | 1 | reservoir | Na-HEPES | 50 (mM) |
