1EFT
THE CRYSTAL STRUCTURE OF ELONGATION FACTOR EF-TU FROM THERMUS AQUATICUS IN THE GTP CONFORMATION
Experimental procedure
Source type | ROTATING ANODE |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 150.700, 99.700, 40.200 |
Unit cell angles | 90.00, 95.30, 90.00 |
Refinement procedure
Resolution | 8.000 - 2.500 |
R-factor | 0.2 |
Rwork | 0.200 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.300 |
Phasing software | X-PLOR |
Refinement software | X-PLOR |
Data quality characteristics
Overall | Outer shell | |
High resolution limit [Å] | 2.200 * | 2.200 * |
Rmerge | 0.450 * | |
Number of reflections | 1443 * | |
Completeness [%] | 74.2 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7.7 * | 19 * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | Tris-HCl | 32 (mM) | |
2 | 1 | drop | 0.5 (mM) | ||
3 | 1 | drop | dithiothreitol | 0.5 (mM) | |
4 | 1 | drop | GDPNP | 0.3 (mM) | |
5 | 1 | drop | AS | 18-22 (%) | |
6 | 1 | drop | EF-Tu.GDPNP | 2.5-5 (mg/ml) | |
7 | 1 | reservoir | Tris-HCl | 32 (mM) | |
8 | 1 | reservoir | 0.5 (mM) | ||
9 | 1 | reservoir | AS | 45-47 |