1EFR
BOVINE MITOCHONDRIAL F1-ATPASE COMPLEXED WITH THE PEPTIDE ANTIBIOTIC EFRAPEPTIN
Experimental procedure
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX9.6 |
Synchrotron site | SRS |
Beamline | PX9.6 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1995-05-04 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 285.700, 107.400, 139.500 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 5.500 - 3.100 |
R-factor | 0.177 * |
Rwork | 0.177 |
R-free | 0.22500 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1bmf |
RMSD bond length | 0.006 |
RMSD bond angle | 16.200 * |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | CCP4 |
Refinement software | TNT |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 5.500 * | 3.350 |
High resolution limit [Å] | 3.100 * | 3.100 |
Rmerge | 0.079 | 0.152 |
Number of reflections | 68961 | |
Completeness [%] | 86.0 | 83 |
Redundancy | 2.6 | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Microdialysis * | 7.2 * | 22-24 * | Lutter, R., (1993) J.Mol.Biol., 229, 787. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | 1 | deuterium oxide | 99.9 (%) | |
10 | 1 | 1 | magnesium chloride | 8 (mM) | |
11 | 1 | 1 | AMP-PNP | 0.500 (mM) | * |
12 | 1 | 1 | ADP | 0.010 (mM) | * |
13 | 1 | 2 | PEG6000 | 9 (%(w/v)) | |
14 | 1 | 2 | 2-mercaptoethanol | 5 (mM) | |
15 | 1 | 2 | * solutions | half of concentration * solutions | |
2 | 1 | 1 | Tris-HCl | 100 (mM) | * |
3 | 1 | 1 | PEG6000 | 14 (%(w/v)) | |
4 | 1 | 1 | sodium chloride | 400 (mM) | * |
5 | 1 | 1 | EDTA | 2 (mM) | * |
6 | 1 | 1 | sodium azide | 0.04 (%(w/v)) | * |
7 | 1 | 1 | PMSF | 0.02 (%(w/v)) | * |
8 | 1 | 1 | dithiothreitol | 10 (mM) | * |
9 | 1 | 2 | magnesium sulphate | 20 (mM) |