1EFP

ELECTRON TRANSFER FLAVOPROTEIN (ETF) FROM PARACOCCUS DENITRIFICANS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	297
Detector technology	IMAGE PLATE
Collection date	1995-01
Detector	RIGAKU RAXIS IIC
Spacegroup name	P 21 21 21
Unit cell lengths	70.430, 80.530, 183.970
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	12.000 - 2.600
R-factor	0.193
Rwork	0.193
R-free	0.28600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1efv
RMSD bond length	0.007
RMSD bond angle	1.500
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.600
Rmerge	0.054
Total number of observations	118452 *
Number of reflections	28666
Completeness [%]	87.8
Redundancy	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5.8	19 *	18% PEG 8000, 25 MM KH2PO4, 100 UM FAD, AND 5 MM MGCL2, FINAL PH = 5.8. THE PROTEIN WAS MIXED WITH SOLUTION IN A 1:1 RATIO (PROTEIN WAS IN 10 MM TRIS, PH 7.4).

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	12 (mg/ml)
2	1	reservoir	PEG8000	18 (%(w/v))
3	1	reservoir	potassium phosphate	25 (mM)
4	1	reservoir	FAD	0.100 (mM)
5	1	reservoir		5 (mM)