1EFO
CRYSTAL STRUCTURE OF AN ADENINE BULGE IN THE RNA CHAIN OF A DNA/RNA HYBRID, D(CTCCTCTTC)/R(GAAGAGAGAG)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 297 |
Detector technology | IMAGE PLATE |
Collection date | 1999-07-22 |
Detector | RIGAKU RAXIS IIC |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 46.660, 47.610, 54.040 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 2.300 |
R-factor | 0.199 |
Rwork | 0.199 |
R-free | 0.22200 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.200 |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 10.000 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.069 * | 0.080 |
Number of reflections | 2578 * | |
Completeness [%] | 91.4 | 71.3 |
Redundancy | 5.6 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | MPD, LITHIUM CHLORIDE, SPERMINE TETRACHLORIDE, CACODYLATE, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | SPERMINE TETRACHLORIDE | ||
2 | 1 | 1 | CACODYLATE | ||
3 | 1 | 1 | LICL | ||
4 | 1 | 1 | MPD | ||
5 | 1 | 2 | LICL | ||
6 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 1 (mM) | |
2 | 1 | drop | 100 (mM) | ||
3 | 1 | drop | spermine tetrachloride | 10 (mM) | |
4 | 1 | drop | sodium cacodylate | 20 (mM) | |
5 | 1 | drop | MPD | 5 (%(v/v)) | |
6 | 1 | reservoir | 40 (%) |