1EFM
STRUCTURE OF THE GDP DOMAIN OF EF-TU AND LOCATION OF THE AMINO ACIDS HOMOLOGOUS TO RAS ONCOGENE PROTEINS
Experimental procedure
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 98.609, 100.807, 162.465 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.6 * | 4,8,14,20 and 22 * | referred to J.Mol.Biol. 185.215-217 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | EF-Tu-GDP | 19 (mg/ml) | trypsin-modified |
| 2 | 1 | drop | Tris-HCl | 50 (mM) | |
| 3 | 1 | drop | 10 (mM) | ||
| 4 | 1 | drop | 2-mercaptoethanol | 10 (mM) | |
| 5 | 1 | drop | GDP | 2 (mM) | |
| 6 | 1 | drop | PEG3350 | 3 (%(w/v)) | |
| 7 | 1 | reservoir | PEG3350 | 30 (%(w/v)) |
