1EFG
THE CRYSTAL STRUCTURE OF ELONGATION FACTOR G COMPLEXED WITH GDP, AT 2.7 ANGSTROMS RESOLUTION
Experimental procedure
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 77.269, 115.447, 106.224 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.700 |
| R-factor | 0.267 |
| Rwork | 0.267 |
| R-free | 0.39600 |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| Number of reflections | 26380 |
| Completeness [%] | 97.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.8 | 277 | pH 7.8, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG8000 | 16-20 (%(w/v)) | |
| 2 | 1 | reservoir | Tris-HCl | 20 (mM) | |
| 3 | 1 | reservoir | dithiothreitol | 2 (mM) | |
| 4 | 1 | reservoir | GDP | 1 (mM) | |
| 5 | 1 | reservoir | 1 (mM) |
