1EF8
CRYSTAL STRUCTURE OF METHYLMALONYL COA DECARBOXYLASE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 200 |
| Detector technology | AREA DETECTOR |
| Collection date | 1998-04-07 |
| Detector | SIEMENS HI-STAR |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 130.300, 115.400, 80.300 |
| Unit cell angles | 90.00, 127.80, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.850 |
| R-factor | 0.18 |
| Rwork | 0.178 |
| R-free | 0.23700 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 2.030 |
| Data scaling software | XDS |
| Refinement software | TNT (5E) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.930 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.022 * | 0.104 |
| Number of reflections | 72476 | |
| <I/σ(I)> | 23.3 | |
| Completeness [%] | 89.0 | 77.2 |
| Redundancy | 2.4 | 1.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Batch method * | 9 | 288 | used microseeding * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | PEG8000 | 3 (%) | |
| 2 | 1 | 1 | ammonium sulfate | 100 (mM) | |
| 3 | 1 | 1 | CHES | 100 (mM) | |
| 4 | 1 | 1 | sodium azide | 5 (mM) |
