1EF7
CRYSTAL STRUCTURE OF HUMAN CATHEPSIN X
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 289 |
Detector technology | IMAGE PLATE |
Collection date | 1999-09-14 |
Detector | MARRESEARCH |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 62.251, 92.480, 209.876 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 2.670 |
R-factor | 0.183 |
R-free | 0.22600 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.600 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | EPMR |
Refinement software | MAIN |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 13.400 | 2.830 |
High resolution limit [Å] | 2.670 | 2.670 |
Rmerge | 0.062 | 0.240 |
Total number of observations | 152861 * | |
Number of reflections | 16732 | |
<I/σ(I)> | 11.9 | |
Completeness [%] | 95.0 | 87 * |
Redundancy | 9.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | PEG 4000, 2-propanol, Na-HEPES, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 8.8 (mg/ml) | |
2 | 1 | drop | Na acetate | 20 (mM) | |
3 | 1 | drop | EDTA | 1 (mM) | |
4 | 1 | reservoir | PEG4000 | 14.4 (%) | |
5 | 1 | reservoir | 2-propanol | 7.2 (%) | |
6 | 1 | reservoir | Na-HEPES | 0.072 (M) |