1EEQ
M4L/Y(27D)D/T94H Mutant of LEN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1998-09-21 |
| Detector | CUSTOM-MADE |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 62.500, 105.200, 42.300 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 7.990 - 1.500 |
| R-factor | 0.211 |
| Rwork | 0.211 |
| R-free | 0.23800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1LVE. Dimer generated by crystallographic operation. Atoms beyond CB removed for mutated residues. |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (0.3C) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.049 | 0.207 |
| Number of reflections | 44489 | |
| <I/σ(I)> | 46.6 | 6.6 |
| Completeness [%] | 98.8 | 95.6 |
| Redundancy | 17 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 20% PEG 3350, 10% 2-propanol, 0.1 M HEPES pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG3350 | 20 (%) | |
| 2 | 1 | reservoir | 2-propanol | 10 (%) | |
| 3 | 1 | reservoir | sodium HEPES | 0.1 (M) | |
| 4 | 1 | drop | protein | 10 (mg/ml) |
