1EEM
GLUTATHIONE TRANSFERASE FROM HOMO SAPIENS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-12-12 |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 57.090, 57.090, 140.280 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.000 |
| Rwork | 0.219 |
| R-free | 0.27100 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.210 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.087 | 0.394 |
| Number of reflections | 17105 | |
| <I/σ(I)> | 17.13 | |
| Completeness [%] | 92.0 | 78.2 |
| Redundancy | 4.3 | 3.09 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 298 | 50mM sodium acetate, 1.0M Ammonium sulfate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | dithiothreitol | 1 (mM) | |
| 2 | 1 | drop | GSH-GSSG | 2 (mM) | |
| 3 | 1 | drop | protein | 8-12 (mg/ml) | |
| 4 | 1 | drop | sodium acetate | 50 (mM) | |
| 5 | 1 | drop | ammonium sulfate | 1.0-1.2 (M) |
