1EEH
UDP-N-ACETYLMURAMOYL-L-ALANINE:D-GLUTAMATE LIGASE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM02 |
Synchrotron site | ESRF |
Beamline | BM02 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 1997-09-01 |
Detector | XRII-CCD |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 58.840, 63.280, 114.910 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 13.000 - 1.900 |
R-factor | 0.219 * |
Rwork | 0.219 |
R-free | 0.26500 |
RMSD bond length | 0.009 |
RMSD bond angle | 23.600 * |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | AMoRE |
Refinement software | CNS (0.9) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 13.392 | 1.940 |
High resolution limit [Å] | 1.878 | 1.880 |
Rmerge | 0.040 * | 0.164 * |
Number of reflections | 31255 | |
<I/σ(I)> | 13 | 3.3 |
Completeness [%] | 87.7 | 49.1 |
Redundancy | 2.5 | 1.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 15 * | PROTEIN SOLUTION: 9.8 mg/ml MURD, 1 mM UMA, 1 mM Sodium Azide, 1 mM DTT, 20 mM HEPES pH 7.5 RESEVOIR: 5-9 % PEG 3350 Monodisperse, 100 mM sodium Acetate pH 5.2, VAPOR DIFFUSION, HANGING DROP, temperature 288K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | MurD | 9.8 (mg/ml) | |
2 | 1 | drop | UMA | 1 (mM) | |
3 | 1 | drop | 1 (mM) | ||
4 | 1 | drop | dithiothreitol | 1 (mM) | |
5 | 1 | drop | HEPES | 20 (mM) | |
6 | 1 | reservoir | PEG3350 monodisperse | 5-9 (%(w/v)) | |
7 | 1 | reservoir | sodium acetate | 100 (mM) |