1EE5

YEAST KARYOPHERIN (IMPORTIN) ALPHA IN A COMPLEX WITH A NUCLEOPLASMIN NLS PEPTIDE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE A1
Synchrotron site	CHESS
Beamline	A1
Temperature [K]	100
Detector technology	CCD
Spacegroup name	C 1 2 1
Unit cell lengths	157.560, 63.700, 62.150
Unit cell angles	90.00, 97.34, 90.00

Refinement procedure

Resolution	30.000 - 2.400
R-factor	0.241 *
Rwork	0.241
R-free	0.26500
RMSD bond length	0.008
RMSD bond angle	3.450
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	30.000
High resolution limit [Å]	2.400	2.400
Rmerge	0.057	0.286
Total number of observations	105706 *
Number of reflections	21907 *
<I/σ(I)>	32.7
Completeness [%]	90.9	80
Redundancy	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7	4 *	pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG8000	16 (%(w/v))
2	1	reservoir		250 (mM)
3	1	reservoir	n-octylglucoside	50 (mM)
4	1	drop	protein	20 (mg/ml)