1EE3
Cadmium-substituted bovine pancreatic carboxypeptidase A (alfa-form) at pH 7.5 and 2 mM chloride in monoclinic crystal form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 291 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-10-15 |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.178, 60.205, 51.715 |
| Unit cell angles | 90.00, 97.46, 90.00 |
Refinement procedure
| Resolution | 40.000 - 1.700 |
| R-factor | 0.174 |
| Rwork | 0.174 |
| R-free | 0.19780 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5CPA.PDB |
| RMSD bond length | 0.005 * |
| RMSD bond angle | 1.280 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.720 |
| High resolution limit [Å] | 1.700 | 1.690 |
| Rmerge | 0.057 | 0.244 |
| Total number of observations | 348864 * | |
| Number of reflections | 31210 | |
| <I/σ(I)> | 29 | 5.4 |
| Completeness [%] | 98.4 | 84.9 |
| Redundancy | 11.4 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICRODIALYSIS | 7.5 | 293 | Lithium Chloride, Tris-HNO3, Cadmium Chloride, pH 7.5, MICRODIALYSIS, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | 1.0 (M) | ||
| 2 | 1 | 1 | Tris-HNO3 | 20 (mM) | pH7.5 |
| 3 | 1 | 1 | protein | 20 (mg/ml) | |
| 4 | 1 | 2 | 0.35 (M) | ||
| 5 | 1 | 2 | Tris-HNO3 | 20 (mM) | pH7.5 |
