1EDY

CRYSTAL STRUCTURE OF RAT ALPHA 1-MACROGLOBULIN RECEPTOR BINDING DOMAIN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	100
Detector technology	CCD
Collection date	1996-10-20
Detector	PRINCETON 2K
Spacegroup name	P 1 21 1
Unit cell lengths	64.377, 36.151, 77.978
Unit cell angles	90.00, 105.88, 90.00

Refinement procedure

Resolution	14.880 - 2.300
R-factor	0.2269
Rwork	0.228
R-free	0.26400
RMSD bond length	0.006
RMSD bond angle	1.278
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (0.9)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.065 *	0.205 *
Number of reflections	14814
<I/σ(I)>	19.5
Completeness [%]	94.6	72.5
Redundancy	3.28	3.28

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	20 *	PEG6000, calcium chloride, hepes, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG6000	22 (%)
2	1	reservoir	Na-HEPES	100 (mM)
3	1	reservoir		25 (mM)