1EDM

EPIDERMAL GROWTH FACTOR-LIKE DOMAIN FROM HUMAN FACTOR IX

Experimental procedure

Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	1992-01-01
Detector	SIEMENS
Spacegroup name	P 43 21 2
Unit cell lengths	40.300, 40.300, 98.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 1.500
R-factor	0.157
Rwork	0.157
Structure solution method	MIR
Starting model (for MR)	NMR MODEL
RMSD bond length	0.010
RMSD bond angle	1.250
Data reduction software	3D-SCALE ((D. STUART))
Data scaling software	XDS
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.650
High resolution limit [Å]	1.500 *	1.500
Rmerge	0.102 *
Total number of observations	48451 *
Number of reflections	13041 *
Completeness [%]	94.5 *	92
Redundancy	3.7 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	7.3	289 *	pH 7.3

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	drop	Tris-HCl	50 (mM)
3	1	drop	calcium chloride	20 (mM)
4	1	reservoir	PEG400	48 (%)