1EDB
CRYSTALLOGRAPHIC AND FLUORESCENCE STUDIES OF THE INTERACTION OF HALOALKANE DEHALOGENASE WITH HALIDE IONS: STUDIES WITH HALIDE COMPOUNDS REVEAL A HALIDE BINDING SITE IN THE ACTIVE SITE
Experimental procedure
Spacegroup name | P 21 21 2 |
Unit cell lengths | 95.400, 72.900, 41.400 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 15.000 - 2.010 |
R-factor | 0.175 |
RMSD bond angle | 3.100 |
Refinement software | TNT |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 9999.000 * |
High resolution limit [Å] | 2.010 * |
Rmerge | 0.067 * |
Total number of observations | 47248 * |
Number of reflections | 16306 * |
Completeness [%] | 89.5 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | referred to J.Mol.Biol. 200.611-612 * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein solution | 0.005ml | |
2 | 1 | drop | ammonium sulfate | 60-70 (%sat) | |
3 | 1 | drop | bis-Tris-H2SO4 | ||
4 | 1 | reservoir | ammonium sulfate | 60-70 (%sat) | |
5 | 1 | reservoir | bis-Tris-H2SO4 |