1ED8
STRUCTURE OF E. COLI ALKALINE PHOSPHATASE INHIBITED BY THE INORGANIC PHOSPHATE AT 1.75A RESOLUTION
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 298 |
| Detector technology | AREA DETECTOR |
| Collection date | 1996-08-10 |
| Detector | UCSD MARK II |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 195.150, 167.340, 76.800 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 1.750 |
| R-factor | 0.154 * |
| Rwork | 0.196 |
| R-free | 0.22800 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 24.700 * |
| Data reduction software | SDMS |
| Data scaling software | SDMS |
| Phasing software | X-PLOR |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 1.880 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.064 | 0.312 |
| Number of reflections | 116581 | |
| <I/σ(I)> | 10.8 | |
| Completeness [%] | 92.5 | 74.5 * |
| Redundancy | 3 | 1.81 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9.5 * | 298 | Tris, ammonium sulfate, magnesium chloride, zinc chloride , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 30 (mg/ml) | |
| 2 | 1 | drop | ammonium sulfate | 20 (%sat) | |
| 3 | 1 | drop | Tris-HCl | 100 (mM) | |
| 4 | 1 | drop | 10 (mM) | ||
| 5 | 1 | drop | 0.01 (mM) | ||
| 6 | 1 | reservoir | ammonium sulfate | 39-43 (%sat) |
