1ED3
CRYSTAL STRUCTURE OF RAT MINOR HISTOCOMPATIBILITY ANTIGEN COMPLEX RT1-AA/MTF-E.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 93 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-07-09 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 86.950, 90.720, 117.410 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.890 - 2.550 |
| R-factor | 0.224 * |
| Rwork | 0.222 |
| R-free | 0.28200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2clr |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.640 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Rmerge | 0.095 | 0.318 |
| Number of reflections | 28978 | 2757 * |
| <I/σ(I)> | 11.4 | 2.6 |
| Completeness [%] | 94.3 | 91.2 |
| Redundancy | 3.2 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.2 * | 17 * | 0.1M morpholine ethansulfonic acid (MES), 0.2M ammonium sulfate, 15-20% MPEG 5000, 0.025M beta-octyl-glucoside, 0.5% glycerol, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 290K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15 (mg/ml) | |
| 2 | 1 | drop | HEPES | 10 (mM) | |
| 3 | 1 | drop | 25 (mM) | ||
| 4 | 1 | drop | EDTA | 1 (mM) | |
| 5 | 1 | reservoir | MES | 0.1 (M) | |
| 6 | 1 | reservoir | ammonium sulfate | 0.2 (M) | |
| 7 | 1 | reservoir | mPEG5000 | 15-20 (%) |
