1ED1

CRYSTAL STRUCTURE OF SIMIAN IMMUNODEFICIENCY VIRUS MATRIX ANTIGEN (SIV MA) AT 100K.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX7.2
Synchrotron site	SRS
Beamline	PX7.2
Temperature [K]	100
Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Spacegroup name	H 3
Unit cell lengths	113.300, 113.300, 31.900
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 2.100
R-factor	0.18 *
Rwork	0.180
R-free	0.24300
RMSD bond length	0.010
RMSD bond angle	1.400
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.100
Rmerge	0.059
Total number of observations	43654 *
Number of reflections	8719
Completeness [%]	97.9
Redundancy	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293	PEG 8000, isopropanol, Tris-HCL, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293.0K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5 (mg/ml)
2	1	drop	PEG3600	9 (%)
3	1	drop	isopropanol	7.5 (%)
4	1	drop	Tris-HCl	25 (mM)
5	1	reservoir	PEG8000	25 (%(w/v))
6	1	reservoir	isopropanol	15 (%(v/v))
7	1	reservoir	Tris-HCl	50 (mM)