1ECY
PROTEASE INHIBITOR ECOTIN
Experimental procedure
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-6A |
Synchrotron site | Photon Factory |
Beamline | BL-6A |
Temperature [K] | 287 |
Detector technology | IMAGE PLATE |
Collection date | 1994-06-24 |
Detector | FUJI |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 63.320, 63.320, 84.130 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 6.000 - 2.190 |
R-factor | 0.213 |
Rwork | 0.213 |
R-free | 0.31900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | ECOTIN PART OF ECOTIN-TRYPSIN COMPLEX |
RMSD bond length | 0.009 |
RMSD bond angle | 26.670 * |
Data reduction software | WEIS |
Data scaling software | WEIS |
Phasing software | X-PLOR (3.1) |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 84.000 | 2.290 |
High resolution limit [Å] | 2.190 | 2.190 |
Rmerge | 0.073 | |
Number of reflections | 8537 | |
Completeness [%] | 91.6 | 73.2 |
Redundancy | 7.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 8.5 * | 22.5-23.5 * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 30 (mg/ml) | |
2 | 1 | drop | ADA | 50 (mM) | |
3 | 1 | drop | Tris-HCl | 50 (mM) | |
4 | 1 | drop | PEG1500 | 20 (%(w/v)) | |
5 | 1 | drop | trehalose | 0.1 (%(w/v)) | |
6 | 1 | reservoir | Tris-HCl | 50 (mM) | |
7 | 1 | reservoir | PEG1500 | 36 (%(w/v)) |