1ECX
NIFS-LIKE PROTEIN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-12-01 |
| Detector | MARRESEARCH |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 115.410, 115.410, 156.950 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.040 - 2.700 |
| R-factor | 0.207 |
| Rwork | 0.207 |
| R-free | 0.25600 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.200 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.040 | 2.870 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.102 | |
| Number of reflections | 33510 | |
| Completeness [%] | 99.3 | 99.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 * | 291 | AMMONIUMSULPHATE, HEPES, DTT, pH 7.8, VAPOR DIFFUSION, SITTING DROP, temperature 18K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10-20 (mg/ml) | |
| 2 | 1 | drop | dithiothreitol | 10 (mM) | |
| 3 | 1 | reservoir | ammonium sulfate | 0.9 (M) | |
| 4 | 1 | reservoir | NaOH/HEPES | 100 (mM) | pH7.5 |
