1ECM
ATOMIC STRUCTURE OF THE BURIED CATALYTIC POCKET OF ESCHERICHIA COLI CHORISMATE MUTASE
Experimental procedure
| Detector technology | IMAGE PLATE |
| Collection date | 1994-02-01 |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 80.830, 80.830, 83.320 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 8.000 - 2.200 |
| R-factor | 0.192 |
| Rwork | 0.192 |
| R-free | 0.23100 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.600 |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| Rmerge | 0.050 |
| Number of reflections | 23622 |
| Completeness [%] | 98.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.85 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein-inhibitor solution | 10.5 (mg/ml) | |
| 2 | 1 | reservoir | sodium cacodylate | 100 (mM) | |
| 3 | 1 | reservoir | sodium acetate | 200 (mM) | |
| 4 | 1 | reservoir | PEG8000 | 28-30 (%) |
