1ECJ
ESCHERICHIA COLI GLUTAMINE PHOSPHORIBOSYLPYROPHOSPHATE (PRPP) AMIDOTRANSFERASE COMPLEXED WITH 2 AMP PER TETRAMER
Experimental procedure
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-6A |
Synchrotron site | Photon Factory |
Beamline | BL-6A |
Temperature [K] | 279 |
Detector technology | IMAGE PLATE |
Collection date | 1992-02 |
Detector | FUJI |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 116.800, 156.000, 106.900 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.500 |
R-factor | 0.181 |
Rwork | 0.181 |
R-free | 0.24800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1gph |
RMSD bond length | 0.008 |
RMSD bond angle | 23.320 * |
Data reduction software | WEIS |
Data scaling software | Agrovata |
Phasing software | GLRF |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.610 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.065 * | |
Total number of observations | 199847 * | |
Number of reflections | 55291 | |
<I/σ(I)> | 37 | 4.3 |
Completeness [%] | 77.8 | 56.1 |
Redundancy | 3.6 | 1.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, sitting drop * | 6.3 | 27 * | 100MM MES, 50MM TRIS, 2MM AMP, 4MM EDTA, 15-18% PEG-3350, PH 6.3 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | MES | 100 (mM) | |
10 | 1 | drop | PEG3350 | 7.5-9 (%(w/v)) | |
11 | 1 | drop | protein | 3.5-17.5 (mg/ml) | |
2 | 1 | reservoir | Tris | 50 (mM) | |
3 | 1 | reservoir | AMP | 2 (mM) | |
4 | 1 | reservoir | EDTA | 4 (mM) | |
5 | 1 | reservoir | PEG3350 | 15-18 (%(w/v)) | |
6 | 1 | drop | MES | 50 (mM) | |
7 | 1 | drop | Tris | 25 (mM) | |
8 | 1 | drop | AMP | 1 (mM) | |
9 | 1 | drop | EDTA | 2 (mM) |