1ECF

ESCHERICHIA COLI GLUTAMINE PHOSPHORIBOSYLPYROPHOSPHATE (PRPP) AMIDOTRANSFERASE

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	110
Detector technology	IMAGE PLATE
Collection date	1995-03-18
Detector	RIGAKU
Spacegroup name	C 2 2 21
Unit cell lengths	116.900, 157.500, 106.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.000
R-factor	0.175
Rwork	0.175
R-free	0.23000
Structure solution method	MOLECULAR REPLACEMENT AND SIR PHASES FROM SEMET PROTEIN
Starting model (for MR)	1gph
RMSD bond length	0.011 *
RMSD bond angle	1.730 *
Data reduction software	HKL ((DENZO))
Data scaling software	HKL
Phasing software	SHARP
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.030
High resolution limit [Å]	2.000	2.000
Rmerge	0.041	0.225
Total number of observations	268969 *
Number of reflections	65480
<I/σ(I)>	24.1	4.5
Completeness [%]	98.2	92.7
Redundancy	4.1	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	6.5 *	20 *	pH 5.6

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	reservoir	PEG3350	15 (%(w/v))
3	1	reservoir	2-propanol	5 (%)
4	1	reservoir	PIPES	100 (mM)
5	1	reservoir		4 (mM)